Journal: bioRxiv

Article Title: Whole-body sorcin invalidation does not cause hypothalamic ER stress nor worsens obesity in C57BL/6 male mice fed a westernized diet

doi: 10.1101/2022.09.07.506981

Figure Lengend Snippet: (A) Representative Western (Immuno)blotting of pSTAT3 (Y705), STAT3 and Sorcin from whole protein lysate isolated from wild-type and sorcin null HEK293 cells expressing LepRb and stimulated with leptin (0-1 ng/mL) as described in methods. (B) Quantification of Western (Immuno)blotting from 3 independent experiments, showing the ratio between pSTAT3/STAT3 for cells stimulated with leptin (0-10 ng/mL); NT = non-transfected cells. Values are means ± SEM. Data were analysed for significance using 1-way ANOVA analysis with Tukey’s multiple comparisons test.

Article Snippet: HEK293 cells, seeded in 6-well plates, were transfected with 2 μg LepRb-HA plasmid, encoding full length clone DNA of Human leptin receptor, transcript variant 1 with C terminal HA tag (Sino-Biologicals, # HG10322-CY), using Xfect ™ Transfection Reagent (Takara Bio) according to manufacturer’s instructions.

Techniques: Western Blot, Isolation, Expressing, Transfection

Journal: Endocrinology

Article Title: Leptin Matures Aspects of Lung Structure and Function in the Ovine Fetus

doi: 10.1210/en.2015-1729

Figure Lengend Snippet: Antibody Table

Article Snippet: Ovine long-form leptin receptor , KLH-conjugated synthetic peptide derived from sheep leptin receptor C terminus , Antileptin receptor , Biorbyt, orb6312 , Rabbit; polyclonal , 2 μg/mL.

Techniques: Sequencing, Derivative Assay

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Estradiol affects extracellular leptin:adiponectin ratio in human breast tissue in vivo.

doi: 10.1210/jc.2014-1129

Figure Lengend Snippet: Figure 3. Tamoxifen significantly decreased leptin and increased adiponectin in normal human breast tissue. A. Postmenopausal women underwent microdialysis sampling of breast tissue (n 18) and subcutaneous (sc) abdominal fat (n 16) before tamoxifen treatment and 6 weeks after treatment. *P .05, **P .01. B. To confirm the in vivo findings, whole normal human breast tissue biopsies were cultured with or without 10-6 M tamoxifen (Tam) for 7 days, and adipokine levels in the culture medium were determined. *P .05 (n 9), ****P .0001 (n 12) compared to controls. C. Normal breast tissue biopsies were cultured ex vivo with or without 10-6 M tamoxifen (Tam). Levels of adipokines and their corresponding receptors (leptin receptor [LepR], adiponectin receptor 1 [AdipoR1], and adiponectin receptor 2 [AdipoR2]) were determined by immunohistochemistry analysis. No differences were detected between treatment groups. Representative tissue sections from each treatment group are shown (scale bars, 20 m).

Article Snippet: Immunohistochemistry Formalin-fixed, paraffin-embedded normal breast tissue biopsies and mouse tumors were cut into 4- m sections, deparaffinized, and subjected to high-temperature antigen retrieval prior to incubating with the following primary antibodies: rabbit antihuman leptin (1 g/ml; GenWay Biotech, San Diego, CA, USA), goat antihuman LepR (20 g/ml; AbD Serotec, Düsseldorf, Germany), goat antimouse leptin (10 g/ml; R&D Systems), goat antimouse LepR (10 g/ml; R&D Systems), rabbit antihuman adiponectin (1 g/ml; Abcam, Cambridge, UK), rabbit antihuman AdipoR1 (5 g/ml; Bioss, London, UK), and rabbit antihuman AdipoR2 (5 g/ml; Bioss).

Techniques: Sampling, In Vivo, Cell Culture, Ex Vivo, Immunohistochemistry

Journal: The Journal of clinical endocrinology and metabolism

Article Title: Estradiol affects extracellular leptin:adiponectin ratio in human breast tissue in vivo.

doi: 10.1210/jc.2014-1129

Figure Lengend Snippet: Figure 4. Increased extracellular leptin and decreased adiponectin in human breast cancer in vivo. A. Eleven postmenopausal breast cancer patients underwent microdialysis sampling of the tumor and normal adjacent breast tissue before surgery. *P .05, **P .01. B. FVB/N mice were oophorectomized and treated with or without estradiol (physiologic level), and MMTV- PyMT mammary cancer cells were injected into the dorsal mammary fat pad. At similar tumor sizes, both groups of mice underwent microdialysis sampling of adipokines in the tumor. *P .05, **P .01 (each group, n 7). C. FVB/N mice were treated as described in Figure 4B. Levels of adipokines and their corresponding receptors (leptin receptor [LepR], adiponectin receptor 1 [AdipoR1], and adiponectin receptor 2 [AdipoR2]) in the MMTV-PyMT tumors were determined by immunohistochemistry analysis. Increased LepR staining was observed in estrogen-exposed tumors. Representative tissue sections from each treatment group are shown (scale bars, 20 m).

Article Snippet: Immunohistochemistry Formalin-fixed, paraffin-embedded normal breast tissue biopsies and mouse tumors were cut into 4- m sections, deparaffinized, and subjected to high-temperature antigen retrieval prior to incubating with the following primary antibodies: rabbit antihuman leptin (1 g/ml; GenWay Biotech, San Diego, CA, USA), goat antihuman LepR (20 g/ml; AbD Serotec, Düsseldorf, Germany), goat antimouse leptin (10 g/ml; R&D Systems), goat antimouse LepR (10 g/ml; R&D Systems), rabbit antihuman adiponectin (1 g/ml; Abcam, Cambridge, UK), rabbit antihuman AdipoR1 (5 g/ml; Bioss, London, UK), and rabbit antihuman AdipoR2 (5 g/ml; Bioss).

Techniques: In Vivo, Sampling, Injection, Immunohistochemistry, Staining